PT3 to ICS conversion

Screenshot of the PT32ICS program.
FLIM image of a HEK cell expressing fluorescently labeled SNARE proteins. The photon trace was recorded in PT3 format, converted with the PT32ICS conversion program, and fitted with the TRI2 software from the University of Oxford (available here). The fluorescence lifetime and intensity images were convoluted in FIJI with a custom macro (available here).

This is freeware and I will not take any responsibility for any results obtained with it. Let me know your success stories, please report any bugs, and cite our work.